Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PDK2 in samples. An antibody specific for PDK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial is an enzyme encoded by the PDK2 gene. It codes for an isozyme of pyruvate dehydrogenase kinase.
To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. The human PDK2 gene encodes a protein with a predicted molecular mass of 46,181 Da that shares 96% identity with that of the rat PDK2 gene. Northern blot analysis showed that the PDK2 message was found in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
